@article {238,
	title = {Sexual reproduction, mating system, chloroplast dynamics and abrupt cell size reduction in seudo-nitzschia pungens from the North Sea (Bacillariophyta)},
	journal = {European Journal of Phycology},
	volume = {40},
	number = {4},
	year = {2005},
	note = {Times Cited: 0ArticleEnglishCited References Count: 64996cf},
	month = {NOV},
	pages = {379-395},
	abstract = {Clonal cultures of Pseudo-nitzschia pungens were isolated at various times from seven sites in the North Sea. During the mitotic cell cycle, the two plate-shaped chloroplasts were girdle-appressed during interphase and mitosis. After cytokinesis, the chloroplasts moved onto the parental valve and remained there during the formation of the new hypovalve and until separation and re-arrangement of the sibling cells within the cell chain had been completed. Clones were almost always heterothallic and cultures of opposite mating type isolated from different localities were compatible. Meiosis I was cytokinetic and accompanied by chloroplast division. Meiosis II involved karyokinesis but not cytokinesis and preceded the rearrangement and contraction of the two gametes. Sexual reproduction involved physiological anisogamy. With one exception, gamete behaviour was clone-specific, gametes being active in clones of one mating type but passive in clones of the other mating type. Auxospore development was accompanied by deposition of a transverse and then a longitudinal perizonium. Infrequently, triploid auxospores and presumably haploid auxospores were produced. The four chloroplasts of diploid auxospores did not divide, and behaved synchronously during the two acytokinetic mitotic cycles accompanying the deposition of the initial thecae. Just before the first division of the initial cell, the chloroplasts shifted onto the valves ( two per valve). The division of the initial cell was not accompanied by chloroplast division and so the two daughter cells received two chloroplasts each. Two modes of abrupt cell size reduction were detected. One occurred during initial cell formation when part of the expanded auxospore aborted. The other pattern was more gradual and was observed in growing cultures; during successive cell divisions a frustule constriction appeared and intensified, one chloroplast split into two, and part of the protoplast aborted. A simple naming system is proposed for mating types in pennate diatoms.},
	keywords = {abrupt size reduction, auxospore formation, auxosporulation, Bacillariophyta, chloroplast, complex bacillariophyceae, delicatissima bacillariophyceae, diatoms, domoic acid production, genus, heterothally, marine diatom, mating system, multiseries bacillariophyceae, pennate diatoms, Pseudo-nitzschia, rapid evolution, sellaphora, sexual reproduction},
	url = {<Go to ISI>://000234151300006},
	author = {Chepurnov, V. A. and Mann, D. G. and Sabbe, K. and Vannerum, K. and Casteleyn, G. and Verleyen, E. and Peperzak, L. and Vyverman, W.}
}
@article {485,
	title = {Morphology, phylogeny, and sexual cycle of Pseudo-nitzschia mannii sp nov (Bacillariophyceae): a pseudo-cryptic species within the P-pseudodelicatissima complex},
	journal = {Phycologia},
	volume = {47},
	number = {5},
	year = {2008},
	note = {350KPTimes Cited:1Cited References Count:44},
	month = {Sep},
	pages = {487-497},
	abstract = {There is increasing evidence that cryptic and pseudo-cryptic species are widespread in several diatom taxa. This is the case with the genus Pseudo-nitzschia, which includes chain-forming pennate diatoms that bloom both in coastal and open ocean waters. In the last years, up to 10 new species have been described on the basis of morphological and molecular investigations, and some of them are only distinguishable on the basis of minute ultrastructural features of their siliceous frustule. We here describe the morphology of the vegetative cell and sexual stages of Pseudo-nitzschia mannii sp. nov., and present an ITS2-based phylogeny to illustrate genetic relationships between P. mannii sp. nov. and the closest congeneric species. On the basis of ribosomal and chloroplastidial gene phylogenies, P. mannii is closely related to P. calliantha, from which it can be differentiated by its slightly wider cells and a different arrangement of the poroid sectors. The analysed strains did not produce the ASP-toxin domoic acid.},
	keywords = {auxosporulation, Cell, cryptic species, delicatissima bacillariophyceae, diatoms, its rdna, mating system, morphology, multiseries, pennate diatoms, phylogeny, pseudo-cryptic diversity, Pseudo-nitzschia, pseudo-nitzschia mannii, Reproduction, ribosomal-rna, sequence alignment, sexual reproduction, size-reduction},
	isbn = {0031-8884},
	url = {<Go to ISI>://000259351600005},
	author = {Amato, A. and Montresor, M.}
}
@article {648,
	title = {Intracellular domoic acid production in Pseudo-nitzschia multistriata isolated from the Gulf of Naples (Tyrrhenian Sea, Italy)},
	journal = {Toxicon},
	volume = {55},
	number = {1},
	year = {2010},
	note = {547WXTimes Cited:0Cited References Count:54},
	month = {Jan},
	pages = {157-161},
	abstract = {Twenty-six Pseudo-nitzschia multistriata cultures were tested for intracellular domoic acid production and fourteen were found to be toxic. Four suboptimal growth conditions were compared with conditions observed to be optimal to explore possible triggers for intracellular domoic acid production. Silica- and phosphate-limitation and low light treatment induced elevated toxin concentrations whereas high temperature appeared to suppress it. Inheritance of the toxin-production ability was investigated by measuring intracellular toxin content in a total of thirty-nine F-1 strains from two different crosses. Results showed radical differences in domoic acid levels among the F-1 offspring from the same parents. (C) 2009 Elsevier Ltd. All rights reserved.},
	keywords = {amino-acid, Batch culture, delicatissima bacillariophyceae, domoic acid, gulf of naples, harmful algal blooms, Light, multiseries, phosphorus-limitation, phylogenetic-relationships, Pseudo-nitzschia multistriata, pungens, seriata bacillariophyceae, silica-limitation, silicate limitation, Temperature, Toxicity, toxin},
	isbn = {0041-0101},
	url = {<Go to ISI>://000273921500020},
	author = {Amato, A. and Ludeking, A. and Kooistra, W. H. C. F.}
}
