@article {250,
	title = {Confirmation of domoic acid production by Pseudo-nitzschia australis (Bacillariophyceae) isolated from Irish waters},
	journal = {Journal of Phycology},
	volume = {38},
	number = {6},
	year = {2002},
	note = {ISSN: 0022-3646Language: EnglishSummary Language: English},
	month = {Dec},
	pages = {1106-1112},
	abstract = {A nonaxenic isolate of the potentially toxic diatom Pseudo-nitzschia australis (Frenguelli) from Irish waters was tested in two separate batch culture experiments. When grown under a low irradiance ( similar to 12 mu mol photons.m super(-) super(2).s super(-); 16:8-h light:dark cycle) for up to 40 days, the culture produced only trace amounts of the neurotoxin domoic acid (DA) during late stationary phase. Growth at a higher irradiance ( similar to 115 mu mol photons.m super(-) super(2).s super(-); 12:12-h light:dark cycle) resulted in DA production starting during late exponential phase and reaching a maximum concentration of 26 pg DA.cell super(-) during late stationary phase. Liquid chromatography coupled to mass spectrometry was used to confirm the identity of DA in the culture. Irradiance and photoperiod could be important factors that contribute directly or indirectly to the control of DA production in P. australis. This is the first record of a DA-producing diatom in Irish waters, and results indicate P. australis may have been the source of DA that has recently contaminated shellfisheries in this area.},
	keywords = {ANE, Bacillariophyceae, bacteria, biochemistry, Biological poisons, biophysics, biosynthesis, chromatography, dangerous organisms, diatoms, domoic acid, effects, Eire, Environmental factors, Fungi and Plants, Irradiance, K 03039 Algae, Light, Light intensity, Liquid, Mass spectroscopy, medicines, neurotoxins, O 1010 Viruses, Photoperiods, Protists, Pseudo-nitzschia australis, Q1 01226 Physiology, Q5 01524 Public health, Seafood, shellfish, toxins, X 24171 Microbial},
	author = {Cusack, C. K. and Bates, S. S. and Quilliam, M. A. and Patching, J. W. and Raine, R.}
}
