@article {393,
	title = {Identification of potentially toxic Pseudo-nitzschia (Bacillariophyceae) in New Zealand coastal waters, using lectins},
	journal = {New Zealand Journal of Marine and Freshwater Research},
	volume = {32},
	number = {4},
	year = {1998},
	note = {Times Cited: 7ArticleEnglishCited References Count: 23153vz},
	month = {DEC},
	pages = {537-544},
	abstract = {Identification of species within the domoic acid-producing diatom genus Pseudo-nitzschia is vital for biotoxin monitoring and decisions regarding shellfish harvesting, particularly when blooms are occurring. Several Pseudo-nitzschia species produce high concentrations of domoic acid per cell, whereas others are non-toxic, but all have similar morphologies using standard brightfield light microscopy. FITC-conjugated lectins discriminated between the New Zealand derived species tested in this study, except for P. delicatissima and P. pseudodelicatissima. These results differ from those reported for Pseudo-nitzschia species isolated from Galician (Spanish) waters, which suggests variability in production of surface sugars by these diatoms depending on geographical origin and/or environmental conditions. Ribosomal RNA-targeted oligonucleotide probes, at present being field trialled in several countries including New Zealand and the United States, are therefore still the preferred method of Pseudo-nitzschia species identification underpinning New Zealand{\textquoteright}s biotoxin risk assessment programmes.},
	keywords = {DNA probes, Electron microscopy, lectins, monitoring, phytoplankton, probes, Pseudo-nitzschia},
	url = {<Go to ISI>://000077855100004},
	author = {Rhodes, L. L.}
}
@article {394,
	title = {Pseudo-nitzschia multistriata (Bacillariophyceae) in New Zealand},
	journal = {New Zealand Journal of Marine and Freshwater Research},
	volume = {34},
	number = {3},
	year = {2000},
	note = {Times Cited: 3ArticleEnglishCited References Count: 15362rp},
	month = {SEP},
	pages = {463-467},
	abstract = {Pseudo-nitzschia multistriata (Takano) Takano has been observed in seawater samples from the North Island and from the northern waters of the South Island, New Zealand, with blooms of >1.0 x 10(6) cells litre(-1) being recorded in December 1997 (austral summer), and minor blooms occurring in both summer and winter 1998 and 1999. The species is notable for its sigmoid form, and for its crossreactivity with oligonucleotide probes targeted at P. australis Frenguelli ribosomal RNA. The species is non-toxic, and for biotoxin monitoring purposes needs to be discriminated from the highly toxic P. australis. It falls in the "delicatissima" complex of Pseudo-nitzschia H. Peragallo species, and electron microscopy shows a close resemblance to P. delicatissima (Cleve) Heidin, except for the lack of a central larger interspace.},
	keywords = {Electron microscopy, identification, oligonucleotide probes, phytoplankton monitoring, Pseudo-nitzschia, targeted fluorescent-probes, waters},
	url = {<Go to ISI>://000089792300006},
	author = {Rhodes, L. L. and Adamson, J. and Scholin, C.}
}
@article {53,
	title = {Interntional accreditation of sandwich hybridisation assay format DNA probes for micro-algae},
	journal = {New Zealand Journal of Marine and Freshwater Research},
	volume = {39},
	year = {2005},
	pages = {1225-1231},
	chapter = {1225},
	author = {Chris Scholin and Katie Ayers and Lesley L. Rhodes and John Tyrrell and Melissa Gladstone}
}
@article {202,
	title = {International accreditation of sandwich hybridisation assay format DNA probes for microalgae},
	journal = {New Zealand Journal of Marine and Freshwater Research},
	volume = {39},
	number = {6},
	year = {2005},
	note = {Times Cited: 0ArticleEnglishCited References Count: 21001ho},
	month = {DEC},
	pages = {1225-1231},
	abstract = {The sandwich hybridisation assay (SHA) is a DNA probe-based method for rapid identification and enumeration of toxic micro-algae which uses species specific oligonucleotide probes targeted at ribosomal RNA. It is suited to fragile micro-algal cells which commonly collapse (luring the fixation stage of sample collection, compromising identification by traditional microscopy. The assay has been available for research for several years, but was validated and accepted for international accreditation for commercial laboratory use in New Zealand in May 2004 (International Accreditation New Zealand: ISO 17025). During the validation of the raphidophyte assay, some discrepancies were noted between SHA cell concentration estimates and traditional light microscope cell counts. Higher SHA estimates were recorded when blooms had collapsed but rRNA was still present in sea water. Conversely, higher traditional cell counts occurred when sample delivery was delayed more than 48 h, presumably owing to degradation of rRNA in the live cultures used for the SHA. SHA cell concentration estimates of the toxic diatom bloom-former Pseudo-nitzschia australis were also compared with whole cell format DNA probe counts and traditional microscope counts; SHA counts were comparable for the three methods tested.},
	keywords = {Algal blooms, big glory bay, DNA probes, fibrocapsa japonica, fibrocapsa-japonica raphidophyceae, heterosigma akashiwo, heterosigma-akashiwo, identification, international accreditation, new-zealand, Pseudo-nitzschia australis, sandwich hybridisation assay},
	url = {<Go to ISI>://000234524400003},
	author = {Ayers, K. and Rhodes, L. L. and Tyrrell, J. and Gladstone, M. and Scholin, C.}
}
