Domoic acid production by Pseudo-nitzschia australis and Pseudo-nitzschia calliantha isolated from North Chile

Publication Type:Journal Article
Year of Publication:2009
Authors:G. Alvarez, Uribe, E., Quijano-Scheggia, S., Lopez-Rivera, A., Marino, C., Blanco, J.
Journal:Harmful Algae
Volume:8
Pagination:938-945
Date Published:Sep
ISBN Number:1568-9883
Keywords:amnesic shellfish poisoning, chile, chinhae bay, Coastal waters, complex bacillariophyceae, domoic acid, identification, morphology, multiseries bacillariophyceae, prince-edward-island, Pseudo-nitzschia australis, pseudo-nitzschia calliantha, pseudo-nitzschia pseudodelicatissima, pseudo-nitzschia subfradulenta, pseudodelicatissima, species bacillariophyceae, west-coast
Abstract:

The morphology and toxicity of the four ubiquitous species belonging to the genus Pseudo-nitzschia found in mixed blooms of phytoplankton from northern Chilean waters were studied. The phytoplankton samples and cultures obtained were identified by scanning electron microscopy, revealing the presence of Pseudo-nitzschia australis, P. calliantha, A pseudodelicatissima and A subfraudulenta. This is the first report of A calliantha in northern Chile. Toxin analyses using the LC-MS method confirmed the presence of domoic acid in P. australis and P. calliantha. Domoic acid was not detected in cultures of P. subfraudulenta. This study therefore confirms P. australis and A calliantha as an unequivocal source of domoic acid in Chilean waters. P. australis is probably the most important producer of amnesic shellfish toxin in view of its domoic acid content. However, more research is needed to evaluate the potential for toxin production in P. pseudodelicatissima. (C) 2009 Elsevier B.V. All rights reserved.

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