Morphology, phylogeny, and sexual cycle of Pseudo-nitzschia mannii sp nov (Bacillariophyceae): a pseudo-cryptic species within the P-pseudodelicatissima complex

Publication Type:Journal Article
Year of Publication:2008
Authors:A. Amato, Montresor M.
Journal:Phycologia
Volume:47
Pagination:487-497
Date Published:Sep
ISBN Number:0031-8884
Keywords:auxosporulation, Cell, cryptic species, delicatissima bacillariophyceae, diatoms, its rdna, mating system, morphology, multiseries, pennate diatoms, phylogeny, pseudo-cryptic diversity, Pseudo-nitzschia, pseudo-nitzschia mannii, Reproduction, ribosomal-rna, sequence alignment, sexual reproduction, size-reduction
Abstract:

There is increasing evidence that cryptic and pseudo-cryptic species are widespread in several diatom taxa. This is the case with the genus Pseudo-nitzschia, which includes chain-forming pennate diatoms that bloom both in coastal and open ocean waters. In the last years, up to 10 new species have been described on the basis of morphological and molecular investigations, and some of them are only distinguishable on the basis of minute ultrastructural features of their siliceous frustule. We here describe the morphology of the vegetative cell and sexual stages of Pseudo-nitzschia mannii sp. nov., and present an ITS2-based phylogeny to illustrate genetic relationships between P. mannii sp. nov. and the closest congeneric species. On the basis of ribosomal and chloroplastidial gene phylogenies, P. mannii is closely related to P. calliantha, from which it can be differentiated by its slightly wider cells and a different arrangement of the poroid sectors. The analysed strains did not produce the ASP-toxin domoic acid.

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