Domoic acid production by Pseudo-nitzschia calliantha Lundholm, Moestrup et Hasle (bacillariophyta) isolated from the Black Sea

Publication Type:Journal Article
Year of Publication:2008
Authors:S. Besiktepe, Ryabushko, L., Ediger, D., Yimaz, D., Zenginer, A., Ryabushko, V., Lee, R.
Journal:Harmful Algae
Volume:7
Pagination:438-442
Date Published:Jun
ISBN Number:1568-9883
Keywords:black sea, confirmation, diatom, domoic acid, harmful algae, king scallop, mortality, multiseries, pecten-maximus, phosphate, phytoplankton, pseudo-nitzschia calliantha, pseudodelicatissima, shellfish, silicate limitation
Abstract:

A species of Pseudo-nitzschia isolated from Sevastopol Bay, Black Sea, was examined for its toxicity. The species was identified as P. calliantha Lundholm, Moestrup et Hasle based on SEM and TEM examination. Domoic acid (DA) was detected in batch culture throughout the growth cycle of P. calliantha. The production of DA by this diatom species was confirmed by fluorenylmethoxycarbonyl (FMOC) derivatization and HPLC-fluorescence method. The cellular DA level was higher in the early exponential phase, with the maximum value of 0.95 pg DA cell(-1). In the stationary phase, the cellular DA levels declined. This is the first record of a DA producing diatom isolated from the Black Sea. (C) 2007 Elsevier B.V. All rights reserved.

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