Genetic relatedness of toxic and nontoxic isolates of the marine pennate diatom Pseudo-nitzschia (Bacillariophyceae): Phylogenetic analysis of 18S rRNA sequences

The nuclear small subunit (SSU) rRNA genes from several marine diatoms, including 2 species that have been responsible for toxic blooms, were amplified from total DNA by the polymerase chain reaction (PCR), and the sequences analysed to determine their genetic relatedness. The isolates investigated include 2 morphologically similar forms of the pennate diatom Pseudonitzschia pungens: (1) P. pungens f. multiseries a known producer of the toxin domoic acid, and (2) P. pungens f. pungens, which is not toxic. Strains of a second toxin-producing species, P. australis, and a nontoxic Thalassiosira species were also included in this study. Phylogenetic analyses of sequences by both distance and parsimony methods clearly distinguished the 2 forms of P. pungens and the 2 Pseudonitszschia species from other diatoms for which sequence data are available. Differences in the nucleotide sequences of the 2 forms of P. pungens permitted the design of PCR primers that allowed discrimination between them. This may prove a valuable tool in identifying toxic and nontoxic forms of closely related and morphologically similar diatom species.